Difference between revisions of "Part:BBa K1065203"
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Cells were induced with 0.5 mM of IPTG. The graohic shows that the induced samples (blue trace) grow slightly slower than the controls (red trace).
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Cells were induced with 0.5 mM of IPTG. The graohic shows that the induced samples (blue trace) grow slightly slower than the controls (red trace).<br/>
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Taking this measures we noticed that the induced colture had a really bad and not normal smell of sulfur and methane so we performed the sulfur test with the lead acetate strips:
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<html><img src="https://static.igem.org/mediawiki/2013/6/6f/Tn-2013_Pspac_zolfo.jpg"/width="300"></html>
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 15:24, 1 October 2013

Efe+Bba_B0015 in pSpac (BBa_K823026)

2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) from Pseudomonas Siringae pv, that catalyzes Ethylene biosynthesis from 2-oxoglutarate. The enzyme was inserted in the IPTG inducible vector pSPAC constructed by the LMU Munich iGEM team 2012 for expression in Bacillus subtilis. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki. If interested in this part please contact us.


NOTE: We used a modified WORKING version of the pSpac vector that has been sent to us from the LMU Munich 2012 team.


Toxicity test

We measured the optical density of cells induced and non induced:

Cells were induced with 0.5 mM of IPTG. The graohic shows that the induced samples (blue trace) grow slightly slower than the controls (red trace).
Taking this measures we noticed that the induced colture had a really bad and not normal smell of sulfur and methane so we performed the sulfur test with the lead acetate strips:

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9491
    Illegal suffix found in sequence at 1224
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9491
    Illegal SpeI site found at 1225
    Illegal PstI site found at 1239
    Illegal NotI site found at 1232
    Illegal NotI site found at 9497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9491
    Illegal BglII site found at 319
    Illegal BglII site found at 7085
    Illegal BamHI site found at 2601
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9491
    Illegal suffix found in sequence at 1225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9491
    Illegal XbaI site found at 9506
    Illegal SpeI site found at 1225
    Illegal PstI site found at 1239
    Illegal NgoMIV site found at 17
    Illegal NgoMIV site found at 4148
    Illegal AgeI site found at 1070
    Illegal AgeI site found at 6696
    Illegal AgeI site found at 7658
    Illegal AgeI site found at 8333
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3972
    Illegal BsaI.rc site found at 5411
    Illegal BsaI.rc site found at 7927
    Illegal SapI site found at 2889
    Illegal SapI.rc site found at 6909