Difference between revisions of "Part:BBa K1132022:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1132022=== | ===Applications of BBa_K1132022=== | ||
| + | After 18 hours, clones containing the plasmid (BBa_J23116-TetR-pTet-RFP) show a leaky basal expression of mRFP. We suppose that the promoter was too weak to express TetR in large quantity. Assembly of a stronger promoter could improve the system and lock the response to an ON/OFF response. | ||
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| + | <br><br>Besides, an experience was done to analyze the effect of the aTc inducer. Result show a visible induction of the red fluorescent protein expression by addition of aTC (60 ng/mL). | ||
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| + | https://static.igem.org/mediawiki/2013/d/de/2022.png | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:29, 1 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
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Applications of BBa_K1132022
After 18 hours, clones containing the plasmid (BBa_J23116-TetR-pTet-RFP) show a leaky basal expression of mRFP. We suppose that the promoter was too weak to express TetR in large quantity. Assembly of a stronger promoter could improve the system and lock the response to an ON/OFF response.
Besides, an experience was done to analyze the effect of the aTc inducer. Result show a visible induction of the red fluorescent protein expression by addition of aTC (60 ng/mL).
User Reviews
UNIQb9d8f18c6fe82a0c-partinfo-00000000-QINU UNIQb9d8f18c6fe82a0c-partinfo-00000001-QINU