Difference between revisions of "Part:BBa K1132023"
Mesnageclem (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1132023 short</partinfo> | <partinfo>BBa_K1132023 short</partinfo> | ||
| − | + | A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : <a href="https://parts.igem.org/Part:BBa_K1132022" >BBa_K1132022</a> | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 12:23, 1 October 2013
strong prom-strong rbs-rbs-tetR-term
A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : <a href="https://parts.igem.org/Part:BBa_K1132022" >BBa_K1132022</a>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]