Difference between revisions of "Part:BBa K1189025"
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This part was primarily used to construct <partinfo>BBa_K1189019</partinfo> in order to allow us to express and purify the protein. | This part was primarily used to construct <partinfo>BBa_K1189019</partinfo> in order to allow us to express and purify the protein. | ||
</p> | </p> | ||
+ | ===Applications of BBa_K1189025=== | ||
+ | This part used primarily for the construction of parts containing the heavy ferritin subunit (<partinfo>BBa_K1189018</partinfo> <partinfo>BBa_K1189019</partinfo> <partinfo>BBa_K1189021</partinfo>). | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 00:37, 1 October 2013
Heavy chain human ferritin
This heavy ferritin chain comes from humans. This part along with light ferritin (BBa_K1189024), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures. The difference between light ferritin is that this chain contains a ferroxidase centre. Protein domains which orient toward the core of ferritin molecules cause the oxidation of intracellular iron from Fe^2+ to Fe^3+ to initiate formation of a ferrihydrite core.
This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.
This part was primarily used to construct BBa_K1189019 in order to allow us to express and purify the protein.
Applications of BBa_K1189025
This part used primarily for the construction of parts containing the heavy ferritin subunit (BBa_K1189018 BBa_K1189019 BBa_K1189021).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]