Difference between revisions of "Part:BBa K1111007:Design"
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===Source=== | ===Source=== | ||
| − | The MMP2 | + | The MMP2 CDS comes from the species Homo sapiens. |
===References=== | ===References=== | ||
Revision as of 21:22, 30 September 2013
MMP2 under the control of the pBAD/araC arabinose inducible promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1645
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The construct was obtained by performing a Gibson reaction on the BBa_I746808 part and MMP2-containing plasmid, after an overlap was added by PCR reaction to both of the elements. For the MMP2 CDS, the PCR not only added an overlap complementary to the iGEM part sequence but also a His tag at the beginning of the protein CDS and a 30bp linker at the end of it, so that it would fold properly when expressed followed by the superfolder GFP sequence.
Source
The MMP2 CDS comes from the species Homo sapiens.