Difference between revisions of "Part:BBa K1111007"
 
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This construct is built with human matrix metalloprotease 2 (MMP2) inserted in a plasmid containing the pBAD/araC arabinose inducible promoter and superfolder GFP. The protein coding sequence (CDS) was cloned in between the two afore-mentioned elements with a linker as well, so that induction of the promoter with arabinose would trigger the expression of an MMP2-GFP protein and its secretion.
 
This construct is built with human matrix metalloprotease 2 (MMP2) inserted in a plasmid containing the pBAD/araC arabinose inducible promoter and superfolder GFP. The protein coding sequence (CDS) was cloned in between the two afore-mentioned elements with a linker as well, so that induction of the promoter with arabinose would trigger the expression of an MMP2-GFP protein and its secretion.
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The goal of this construct was to give bacteria the ability to secrete a gelatine-degrading enzyme, upon arabinose signaling (chosen for the proof of principle), to break open gelatin nano-particles containing drugs and attached to the bacteria's surface. In the future, different promoters with desired specificities could be cloned in front of the MMP2-GFP, instead oft he arabinose promoter, to trigger release of drugs upon special signals.
  
 
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Latest revision as of 21:17, 30 September 2013

MMP2 under the control of the pBAD/araC arabinose inducible promoter

This construct is built with human matrix metalloprotease 2 (MMP2) inserted in a plasmid containing the pBAD/araC arabinose inducible promoter and superfolder GFP. The protein coding sequence (CDS) was cloned in between the two afore-mentioned elements with a linker as well, so that induction of the promoter with arabinose would trigger the expression of an MMP2-GFP protein and its secretion.

The goal of this construct was to give bacteria the ability to secrete a gelatine-degrading enzyme, upon arabinose signaling (chosen for the proof of principle), to break open gelatin nano-particles containing drugs and attached to the bacteria's surface. In the future, different promoters with desired specificities could be cloned in front of the MMP2-GFP, instead oft he arabinose promoter, to trigger release of drugs upon special signals.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1645
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961