Difference between revisions of "Part:BBa K1151036"

Revision as of 15:42, 30 September 2013

Double generator NikR-GFP, IPTG-nickel regulated

A simple construct composed of the parts K1151006 + K1151009.

Fluorescence decay assay

The experiment

We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel to BL21 cells transformed with the plasmid containing this part. Then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel added: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 818
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 818
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 818
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 818
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 818
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1616

@@ Line 10: / Line 10: @@
 ''The experiment''
-We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel added: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).
+We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel to BL21 cells transformed with the plasmid containing this part. Then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel added: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).
 [[File:1036.2.jpg]]                          [[File:1036.3.jpg]]