Difference between revisions of "Part:BBa K1151036"

(Fluorescence decay assay)
(Fluorescence decay assay)
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''The experiment''
 
''The experiment''
  
We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel aggiunto: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).
+
We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel added: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).
  
 
[[File:1036.2.jpg]]                          [[File:1036.3.jpg]]
 
[[File:1036.2.jpg]]                          [[File:1036.3.jpg]]

Revision as of 13:10, 30 September 2013

Double generator NikR-GFP, IPTG-nickel regulated

A simple construct composed of the parts K1151006 + K1151009.


Fluorescence decay assay

The experiment

We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel added: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).

1036.2.jpg 1036.3.jpg


Decayas.jpg Gfp2.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 818
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 818
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 818
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1616