Difference between revisions of "Part:BBa K1111004:Experience"
GigiGoesGugu (Talk | contribs) (→Sequencing) |
GigiGoesGugu (Talk | contribs) (→Sequencing) |
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The sequencing result showed that there were no mutations in the insert | The sequencing result showed that there were no mutations in the insert | ||
| − | [[Image: CADSequ.jpg|thumb|600px|center|Figure 1: | + | [[Image: CADSequ.jpg|thumb|600px|center|Figure 1: Sequencing result of the Cad promoter using one foreward Primer that binds directly to the beginning of the promoter ]] |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 11:13, 30 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1111004
We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
Sequencing
The sequencing result showed that there were no mutations in the insert
User Reviews
UNIQdb98f3e1714b8479-partinfo-00000000-QINU UNIQdb98f3e1714b8479-partinfo-00000001-QINU