Difference between revisions of "Part:BBa K1060012"
(italics and addition to const expr MS)
 
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<partinfo>BBa_K1060012 short</partinfo>
 
<partinfo>BBa_K1060012 short</partinfo>
  
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KU_Leuven 2013 requested the <partinfo>BBa_J45700</partinfo> brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (<partinfo>BBa_K1060003</partinfo>). In this part we removed the lac promoter in front of the pchBA gene to get a constitutive expression of all genes needed for methyl salicylate production.  
+
KU_Leuven 2013 requested the <partinfo>BBa_J45700</partinfo> brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (<partinfo>BBa_K1060003</partinfo>). In this part we removed the ''lac'' promoter in front of the ''pchBA'' gene to get a constitutive expression of all genes needed for methyl salicylate production in the absence of the TetR repressor.  
  
  

Latest revision as of 09:47, 30 September 2013

Methyl Salicylate Production under TetR regulation

KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (BBa_K1060003). In this part we removed the lac promoter in front of the pchBA gene to get a constitutive expression of all genes needed for methyl salicylate production in the absence of the TetR repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1656
    Illegal NgoMIV site found at 2060
    Illegal NgoMIV site found at 2185
    Illegal NgoMIV site found at 2196
    Illegal NgoMIV site found at 2477
    Illegal NgoMIV site found at 2945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287