Difference between revisions of "Part:BBa K1111004:Experience"
GigiGoesGugu (Talk | contribs) (→Applications of BBa_K1111004) |
GigiGoesGugu (Talk | contribs) (→Applications of BBa_K1111004) |
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Score Expect Identities Gaps Strand | Score Expect Identities Gaps Strand | ||
893 bits(483) 0.0 483/483(100%) 0/483(0%) Plus/Plus | 893 bits(483) 0.0 483/483(100%) 0/483(0%) Plus/Plus | ||
| − | Query 1 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 60 | + | Query 1 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 60 <BR> |
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| − | Sbjct 72 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 131 | + | Sbjct 72 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 131 <BR> |
Query 61 ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT 120 | Query 61 ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT 120 | ||
Revision as of 09:32, 30 September 2013
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Applications of BBa_K1111004
We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
Score Expect Identities Gaps Strand
893 bits(483) 0.0 483/483(100%) 0/483(0%) Plus/Plus
Query 1 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 72 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 131
Query 61 ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 132 ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT 191
Query 121 GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 192 GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC 251
Query 181 CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 252 CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA 311
Query 241 AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 312 AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT 371
Query 301 GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 372 GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA 431
Query 361 AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 432 AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC 491
Query 421 GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 492 GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC 551
Query 481 GAT 483
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Sbjct 552 GAT 554
User Reviews
UNIQ812405e2d9ae144e-partinfo-00000000-QINU UNIQ812405e2d9ae144e-partinfo-00000001-QINU