Difference between revisions of "Part:BBa K1111004:Experience"

(→‎Applications of BBa_K1111004)
(→‎Applications of BBa_K1111004)
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We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
 
We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
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<BR>
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Score Expect Identities Gaps Strand
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893 bits(483) 0.0 483/483(100%) 0/483(0%) Plus/Plus
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Query  1    CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA  60
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  72  CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA  131
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 +
Query  61  ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT  120
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  132  ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT  191
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 +
Query  121  GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC  180
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  192  GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC  251
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Query  181  CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA  240
 +
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  252  CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA  311
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Query  241  AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT  300
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            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  312  AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT  371
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Query  301  GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA  360
 +
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  372  GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA  431
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 +
Query  361  AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC  420
 +
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 +
Sbjct  432  AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC  491
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 +
Query  421  GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC  480
 +
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Sbjct  492  GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC  551
 +
 +
Query  481  GAT  483
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            |||
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Sbjct  552  GAT  554
  
 
===User Reviews===
 
===User Reviews===

Revision as of 09:32, 30 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1111004

We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.

Score Expect Identities Gaps Strand 893 bits(483) 0.0 483/483(100%) 0/483(0%) Plus/Plus Query 1 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 60 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 72 CCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGA 131

Query 61 ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT 120 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 132 ATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCT 191

Query 121 GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC 180 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 192 GAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCAC 251

Query 181 CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA 240 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 252 CATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCA 311

Query 241 AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT 300 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 312 AAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTT 371

Query 301 GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA 360 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 372 GTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGA 431

Query 361 AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC 420 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 432 AGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGAC 491

Query 421 GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC 480 

           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 492 GTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGC 551

Query 481 GAT 483 

           |||

Sbjct 552 GAT 554

User Reviews

UNIQ8b7245a101a46ae9-partinfo-00000000-QINU UNIQ8b7245a101a46ae9-partinfo-00000001-QINU