Difference between revisions of "Part:BBa K1060011"

 
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<partinfo>BBa_K1060011 short</partinfo>
 
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For the production of the alarmhormone,(E)-&#946;-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006). Additionally a Lac operator sequence (Gilbert & Maxam, 1973) was introduced to regulate its expression.
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For the production of the alarmhormone,(E)-&#946;-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (<partinfo>BBa_K608006</partinfo>). Additionally a Lac operator sequence (Gilbert & Maxam, 1973) was introduced to regulate its expression.
  
 
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Revision as of 09:08, 30 September 2013

Beta Farnesene production

For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006). Additionally a Lac operator sequence (Gilbert & Maxam, 1973) was introduced to regulate its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1409
    Illegal BamHI site found at 709
    Illegal XhoI site found at 671
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 281