Difference between revisions of "Part:BBa K1045002"

Revision as of 08:49, 30 September 2013

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

This part is a reporter system.
It was constructed by amplification of a riboswitch from Bacillus subtilis genomic DNA with a native promoter and native RBS (BBa_K1045004) and combined to a CFP reporter (BBa_E0020). It works as a sensor of c-di-AMP, which enhances the terminating efficiency in the translation of the RNA by ribosomes. The system uses the terminator present in the pSB1C3 backbone. Altogether in the presence of c-di-AMP the system should be hindered and not be glowing. Furthermore, in combination with the diadenylate cyclase domain (like a further recombined part BBa_K1045003), it can report the state of the c-di-AMP pathway. This can be exploited in order to screen substances for effects on c-di-AMP homeostasis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This part is a reporter system.<br />
 It was constructed by amplification of a riboswitch from ''Bacillus subtilis'' genomic DNA with a native promoter and native RBS ([[Part:BBa_K1045004|BBa_K1045004]]) and combined to a CFP reporter ([[Part:BBa_E0020|BBa_E0020]]). It works as a sensor of c-di-AMP, which enhances the terminating efficiency in the translation of the RNA by ribosomes. The system uses the terminator present in the '''pSB1C3''' backbone. Altogether in the presence of c-di-AMP the system should be hindered and not be glowing.
-Furthermore, in combination with the diadenylate cyclase domain (like a further recombined part [[Part:BBa_K1045003|BBa_K1045003]]) it might show further interaction. The diadenylate cyclase domain can have the competence to likewise hinder the system, which turns out with no illumination.
+Furthermore, in combination with the diadenylate cyclase domain (like a further recombined part [[Part:BBa_K1045003|BBa_K1045003]]), it can report the state of the c-di-AMP pathway. This can be exploited in order to screen substances for effects on c-di-AMP homeostasis.
 <!-- Add more about the biology of this part here