Difference between revisions of "Part:BBa K1127001"

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A3-Flg is a gold binding peptide. To stabilize it in vivo it was fused with IM9 protein.
 
A3-Flg is a gold binding peptide. To stabilize it in vivo it was fused with IM9 protein.
  
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==Characterization==
 
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We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [https://parts.igem.org/Part:BBa_J23100] which allows constitutive expression of the peptide.
 
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We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127001/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].
 
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[[File:A3-Flg IM9.mp4]]
 
  
 
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Latest revision as of 00:01, 30 September 2013

A3-Flg IM9 fusion part

A3-Flg is a gold binding peptide. To stabilize it in vivo it was fused with IM9 protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]