Difference between revisions of "Part:BBa K1127000"

Revision as of 00:01, 30 September 2013

A3-Flg Gold binding peptide

A de novo synthesized gene with A3 and Flg domains which should hypothetically bind to gold and form nanoparticles.

Characterization

We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [1] which allows constitutive expression of the peptide.

We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127000/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].

File:A3-Flg IM9.mp4 File:PelB-A3-Flg- STOP.mp4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 78
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ==Characterization==
+We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [https://parts.igem.org/Part:BBa_J23100] which allows constitutive expression of the peptide.
+We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127000/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].
+[[File:A3-Flg IM9.mp4]]
 [[File:PelB-A3-Flg- STOP.mp4|center]]