Difference between revisions of "Part:BBa K1127003"

Revision as of 23:58, 29 September 2013

MIDAS2 gold binding peptide

MIDAS2 is a gold binding synthetic peptide. We synthesized a gene expressing this peptide with a pelB signaling sequence to be excreted out of the E. coli.

Characterization

We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [1] which allows constitutive expression of the peptide.

We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127003/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].

File:MIDAS2 gold binding peptide.mp4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 78
Illegal AgeI site found at 91
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1127003 short</partinfo>
@@ Line 5: / Line 4: @@
 MIDAS2 is a gold binding synthetic peptide. We synthesized a gene expressing this peptide with a pelB signaling sequence to be excreted out of the E. coli.
-<!-- Add more about the biology of this part here
+==Characterization==
-===Usage and Biology===
+We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [https://parts.igem.org/Part:BBa_J23100] which allows constitutive expression of the peptide.
+We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127003/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].
+[[File:MIDAS2 gold binding peptide.mp4]]
 <!-- -->