Difference between revisions of "Part:BBa K1127002"

Revision as of 23:55, 29 September 2013

IM9 and A3-Flg fusion for gold binding

This is the inverted version of the part BBa_K1127020 with the same function.

Characterization

We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [1] which allows constitutive expression of the peptide.

We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127002/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].

File:MIDAS2 gold binding peptide.mp4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 78
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127002/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].
-[[File:PelB-IM9-Link-A3-Flg-_STOP.mp4]]
+[[File:MIDAS2 gold binding peptide.mp4]]
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