Difference between revisions of "Part:BBa K1189031:Design"

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===Design Notes===
 
===Design Notes===
 
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<p>This sequence was optimized for expression in <i>E. coli K12</i>. As opposed to the eukaryotic Kozak seuquence on <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">BBa_782004 TALE A</a> from which the TALE in this sequence was inspired, the <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> team placed a strong prokaryotic (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>) RBS to express it.</p>
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<p>This sequence was optimized for expression in <i>E. coli K12</i>. As opposed to the eukaryotic Kozak seuquence on <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">BBa_782004 TALE A</a> from which the TALE in this sequence was inspired, the <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> team placed a strong prokaryotic (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>) RBS to express it. A GGGS*4 linker was placed between the two protein domains to prevent steric hindrance between the two functional domains. The KasI restriction site on the C terminus of the TALE and the N terminus of beta-lactamase is designed to allow user switch in new promoter-RBS-TALE sequences to improve modularity of our DNA detection system. Depending on the TALE sequence selected, on can detect any sort of DNA sequence of interest.</p>
  
 
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Latest revision as of 23:13, 29 September 2013


TALE-A linked to beta-lactamase with a his tag under a lacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1922
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2651
    Illegal AgeI site found at 677
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized for expression in E. coli K12. As opposed to the eukaryotic Kozak seuquence on BBa_782004 TALE A from which the TALE in this sequence was inspired, the iGEM Calgary 2013 team placed a strong prokaryotic (B0034) RBS to express it. A GGGS*4 linker was placed between the two protein domains to prevent steric hindrance between the two functional domains. The KasI restriction site on the C terminus of the TALE and the N terminus of beta-lactamase is designed to allow user switch in new promoter-RBS-TALE sequences to improve modularity of our DNA detection system. Depending on the TALE sequence selected, on can detect any sort of DNA sequence of interest.

Source

This sequence was generated synthetically by Genscript.

References