Difference between revisions of "Part:BBa K1189031:Design"

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===Design Notes===
 
===Design Notes===
 
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<p>This sequence was generated synthetically by <a href="http://www.genscript.com/">Genscript</a> and optimized for <i>E. coli K12</i>. As opposed to the eukaryotic Kozak seuquence on <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">BBa_782004 TALE A</a> from which the TALE in this sequence was inspired, the <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> team placed a strong prokaryotic (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>) RBS to express it.</p>
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<p>This sequence was optimized for expression in <i>E. coli K12</i>. As opposed to the eukaryotic Kozak seuquence on <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">BBa_782004 TALE A</a> from which the TALE in this sequence was inspired, the <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> team placed a strong prokaryotic (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>) RBS to express it.</p>
  
 
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Revision as of 23:08, 29 September 2013


TALE-A linked to beta-lactamase with a his tag under a lacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1922
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2651
    Illegal AgeI site found at 677
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized for expression in E. coli K12. As opposed to the eukaryotic Kozak seuquence on BBa_782004 TALE A from which the TALE in this sequence was inspired, the iGEM Calgary 2013 team placed a strong prokaryotic (B0034) RBS to express it.

Source

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References