Difference between revisions of "Part:BBa K1189031"
| Line 7: | Line 7: | ||
<p>This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification.</p> | <p>This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification.</p> | ||
| − | <a>TALE A was inspired by the award winning <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">TALE A</a> from the award winning <a href="http://2012.igem.org/Team:Slovenia">2012 Slovenian iGEM project</a>. The <a href="2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> used this TALE and its associated DNA binding sequence to build a proof of concept TALE based DNA detector. In the case of <a href="https://parts.igem.org/Part:BBa_K1189031">BBa_K1189031</a>, the Calgary team used beta-lactamase as a reporter enzyme to indicate when the TALE is bound to DNA. </p> | + | <a>TALE A was inspired by the award winning <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">TALE A</a> from the award winning <a href="http://2012.igem.org/Team:Slovenia">2012 Slovenian iGEM project</a>. The <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> used this TALE and its associated DNA binding sequence to build a proof of concept TALE based DNA detector. In the case of <a href="https://parts.igem.org/Part:BBa_K1189031">BBa_K1189031</a>, the Calgary team used beta-lactamase as a reporter enzyme to indicate when the TALE is bound to DNA. </p> |
<figure> | <figure> | ||
Revision as of 22:58, 29 September 2013
TALE-A linked to beta-lactamase with a his tag under a lacI promoter
This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification.
TALE A was inspired by the award winning TALE A from the award winning 2012 Slovenian iGEM project. The iGEM Calgary 2013 used this TALE and its associated DNA binding sequence to build a proof of concept TALE based DNA detector. In the case of BBa_K1189031, the Calgary team used beta-lactamase as a reporter enzyme to indicate when the TALE is bound to DNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1922
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2651
Illegal AgeI site found at 677
Illegal AgeI site found at 1258 - 1000COMPATIBLE WITH RFC[1000]