Difference between revisions of "Part:BBa K1127006"

 
 
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<partinfo>BBa_K1127006 short</partinfo>
 
<partinfo>BBa_K1127006 short</partinfo>
  
 
Proteins A B and C of mtr complex put together. B and C complexes have rbs for each, mtrA however does not which allows selected transcription of this protein.
 
Proteins A B and C of mtr complex put together. B and C complexes have rbs for each, mtrA however does not which allows selected transcription of this protein.
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We optimized codon usage of the mtrA for efficient expression within E. coli. In addition, we removed legal sites from the mtrABC sequences (e.g. PstI in the mtrC). Please go to our iGEM wiki [http://2013.igem.org/Team:York_UK/Notebook.html?page=wetlab] for more information.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:28, 29 September 2013

mtrABC electron pump

Proteins A B and C of mtr complex put together. B and C complexes have rbs for each, mtrA however does not which allows selected transcription of this protein.

We optimized codon usage of the mtrA for efficient expression within E. coli. In addition, we removed legal sites from the mtrABC sequences (e.g. PstI in the mtrC). Please go to our iGEM wiki [http://2013.igem.org/Team:York_UK/Notebook.html?page=wetlab] for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3387