Difference between revisions of "Part:BBa K1223011:Design"

(→‎Design Notes)
(→‎References)
Line 18: Line 18:
  
 
===References===
 
===References===
 +
[http://en.wikipedia.org/wiki/Beta-lactamase]

Revision as of 18:56, 29 September 2013


ampR translational unit (ampicilin resistance CDS+promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 154


Design Notes

AmpR gene encodes for the beta-lactamase (EC 3.5.2.6) protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).

this construct is widely used for selection of transformants. this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.

Source

part extracted from pKD4 plasmid by PCR.

References

[http://en.wikipedia.org/wiki/Beta-lactamase]