Difference between revisions of "Part:BBa K1223006:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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purification and identification of protein of interest.
 
  
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The His-Tag (or 6XHis) is a widely use sequence both as a reporter (using anti-His tag antibodies such as His-probe) and as purification tag.
  
 +
the 6 histidine sequence has a very high affinity to nickel(II) ions and therefore is used in affinity purification columns with immobilized nickel ions. the binding of the His-Tag to the nickel ions is not efficient at acidic pH values so any purification process should be done above pH=7 (usually done at pH=8).
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 +
His-Tag binding resins are commercially available and are widely used in recombinant protein purification processes.
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 +
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this part contains the 6XHis sequence between two valine residues and terminated with the TAA stop codon.
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the part can be used at the C-Terminus of the CDS with its included stop codon.
  
 
===Source===
 
===Source===

Revision as of 16:43, 29 September 2013


His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The His-Tag (or 6XHis) is a widely use sequence both as a reporter (using anti-His tag antibodies such as His-probe) and as purification tag.

the 6 histidine sequence has a very high affinity to nickel(II) ions and therefore is used in affinity purification columns with immobilized nickel ions. the binding of the His-Tag to the nickel ions is not efficient at acidic pH values so any purification process should be done above pH=7 (usually done at pH=8).

His-Tag binding resins are commercially available and are widely used in recombinant protein purification processes.


this part contains the 6XHis sequence between two valine residues and terminated with the TAA stop codon.

the part can be used at the C-Terminus of the CDS with its included stop codon.

Source

synthetic

References