Difference between revisions of "Part:BBa K1111002"

 
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<partinfo>BBa_K1111002 short</partinfo>
 
<partinfo>BBa_K1111002 short</partinfo>
  
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This Promoter should be induced by low pH and make the cells express superfolded GFP.
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==Introduction==
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The hya promoter is encoded in the genome of K-12 MG1655 E.Coli bacteria. Upon external acidification it is activated and induced transcribtion of hydrogenase isoenzyme 1. This enzyme can then catalyze reversible oxidation of hydrogen.
  
 
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Revision as of 16:28, 29 September 2013

Hya promoter and Superfolded GFP

Introduction

The hya promoter is encoded in the genome of K-12 MG1655 E.Coli bacteria. Upon external acidification it is activated and induced transcribtion of hydrogenase isoenzyme 1. This enzyme can then catalyze reversible oxidation of hydrogen.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 184
    Illegal NheI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 184
    Illegal AgeI site found at 26
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 543