Difference between revisions of "Part:BBa K1111004"
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==Introduction== | ==Introduction== | ||
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| + | The Cad Promoter was originally discovered in the K-12 E.coli substrain MG1655. It's original purpose is to induce Expression of Lysine decarboxylase. This Enzyme can remove carboxy Groups from acids, thus increasing the external pH of the medium. Thus the promoter is induced upon external acidification and excess Lysine and reaches Maximum Expression under anaerobic conditions. | ||
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Revision as of 14:59, 29 September 2013
superfolded GFP driven by CAD promoter
This constructs expresses GFP when the pH is lowered and there is a high lysine concentration.
Introduction
The Cad Promoter was originally discovered in the K-12 E.coli substrain MG1655. It's original purpose is to induce Expression of Lysine decarboxylase. This Enzyme can remove carboxy Groups from acids, thus increasing the external pH of the medium. Thus the promoter is induced upon external acidification and excess Lysine and reaches Maximum Expression under anaerobic conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 496
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 474
Illegal SapI.rc site found at 540