Difference between revisions of "Part:BBa K1084001"

Revision as of 03:11, 29 September 2013

promoter 1

iGEM Hokkaido_U Japan 2013 original promoter family. Constructed from consensus promoter (BBa_K1084001) by adding mutation at -35 region. Other region is same among family members, and similar with promoter consensus sequence.

Correspondence of sequence and parts number is below.

  -35        BBa_         t. e. rank
  TTGACA     K1084001     1   
  TAGGTC     K1084002     2
  CTGAAG     K1084003     6
  GGGGTG     K1084004     3
  GAGGAT     K1084005     5
  GCAATA     K1084006     7
  GGGGGG     K1084007     8
  TGTGTG     K1084008     4
  AGTGGG     K1084009     9
  TCTCGG     K1084010     10

t. e. = transcription efficyency Promoter transcription efficiency was measured with mRFP1, LacZ and Kanamycine resistance.

Fig. 2 mRFP1 assay result

Fig. 3 β-Galactosidase assay result

Fig. 4 Comparison of assay results and modeling data

Theoretical promoter modeling also has done (see promoter Background page in Hokkaido_U 2013 wiki) According to protein, these promoters show relatively another protein activity revel. Choose your best choise by promoter optimization kit (POK).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 iGEM Hokkaido_U Japan 2013 original promoter family. Constructed from consensus promoter (BBa_K1084001) by adding mutation at -35 region. Other region is same among family members, and similar with promoter consensus sequence.
-"https://static.igem.org/mediawiki/2013/c/c1/HokkaidoU2013_promoter_Result-fig1.png"
+https://static.igem.org/mediawiki/2013/c/c1/HokkaidoU2013_promoter_Result-fig1.png
-Promoter transcription efficiency was measured with mRFP1, LacZ and Kanamycine resistance.
+Correspondence of sequence and parts number is below.
-"https://static.igem.org/mediawiki/2013/7/73/HokkaidoU2013_promoter_Result-fig2.png"
+   -35        BBa_         t. e. rank
-   <div>Fig. 2  mRFP1 assay result</div>
+   TTGACA     K1084001     1
+   TAGGTC     K1084002     2
-"https://static.igem.org/mediawiki/2013/1/1d/HokkaidoU2013_promoter_Result-fig4.png
+   CTGAAG     K1084003     6
-  <div>Fig. 4  &beta;-Galactosidase assay result</div>
+   GGGGTG     K1084004     3
+   GAGGAT     K1084005     5
-"https://static.igem.org/mediawiki/2013/e/eb/HokkaidoU2013_promoter_Result-fig5.png
+   GCAATA     K1084006     7
-  <div>Fig. 5  Comparison of assay results and modeling data</div>
+   GGGGGG     K1084007     8
+   TGTGTG     K1084008     4
+   AGTGGG     K1084009     9
+   TCTCGG     K1084010     10
+t. e. = transcription efficyency
+Promoter transcription efficiency was measured with mRFP1, LacZ and Kanamycine resistance.
+https://static.igem.org/mediawiki/2013/7/73/HokkaidoU2013_promoter_Result-fig2.png
+<div>Fig. 2  mRFP1 assay result</div>
+https://static.igem.org/mediawiki/2013/1/1d/HokkaidoU2013_promoter_Result-fig4.png
+<div>Fig. 3  &beta;-Galactosidase assay result</div>
+https://static.igem.org/mediawiki/2013/e/eb/HokkaidoU2013_promoter_Result-fig5.png
+<div>Fig. 4  Comparison of assay results and modeling data</div>
 Theoretical promoter modeling also has done (see promoter Background page in Hokkaido_U 2013 wiki)
 According to protein, these promoters show relatively another protein activity revel.
 Choose your best choise by promoter optimization kit (POK).