Difference between revisions of "Part:BBa K1150032"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150032 short</partinfo>
 
<partinfo>BBa_K1150032 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|uniCAS UV Light Switch
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Part II - Histone Modificator
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|-
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|'''Function'''
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|Histone modification domain of UVB light
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induced gene expression control
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|-
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|'''Use in'''
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|Mammalians
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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==Introduction==
 
==Introduction==
 
This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation
 
This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation
  
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== References ==
 
−
Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
 
  
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<span class='h3bb'>
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<!-- Add more about the biology of this part here
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==Sequence and Features==
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===Usage and Biology===
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</span>
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1150032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1150032 SequenceAndFeatures</partinfo>
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 +
== References ==
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Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
  
  

Revision as of 23:46, 28 September 2013

uniCAS UV Light Switch Part II - Histone Modifier

uniCAS UV Light Switch

Part II - Histone Modificator

Function Histone modification domain of UVB light

induced gene expression control

Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Introduction

This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 2231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1373
    Illegal BsaI.rc site found at 1450
    Illegal SapI.rc site found at 962

References

Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling