Difference between revisions of "Part:BBa K1067002"

Revision as of 15:51, 28 September 2013

Cytochrome A and X from Nitrosomonas europaea

Cytochrome A (c-554) and cytochrome X (Probable tetraheme cytochrome c-type) both from Nitrosomonas europaea.

The two cytochromes are involved in the process of transferring electrons from HAO to AMO in the following reactions:

HAO reaction, 2 electrons gained:

NH₂OH + H₂O + 2 cycAX_ox → NO₂^- + 2 cycAX_red + 5 H⁺

AMO reaction 2 electrons used:

NH₃ + O₂ + cycAX_red + 2 H⁺ → NH₂OH + H₂O + cycAX_ox

Usage and Biology

When highly expressed in E.coli the cells turn dark red. If grown on media with supplementation of d-aminolevulinic acid (ALA) it tends to get a darker color because of the extra heme incorporated.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 686
Illegal EcoRI site found at 838
Illegal PstI site found at 51
Illegal PstI site found at 490
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 686
Illegal EcoRI site found at 838
Illegal PstI site found at 51
Illegal PstI site found at 490
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 686
Illegal EcoRI site found at 838
Illegal BglII site found at 448
Illegal BamHI site found at 235
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 686
Illegal EcoRI site found at 838
Illegal PstI site found at 51
Illegal PstI site found at 490
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 686
Illegal EcoRI site found at 838
Illegal PstI site found at 51
Illegal PstI site found at 490
Illegal NgoMIV site found at 1140
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Cytochrome A (c-554) and cytochrome X (Probable tetraheme cytochrome c-type) both from ''Nitrosomonas europaea''.
-===Usage and Biology===
 The two cytochromes are involved in the process of transferring electrons from HAO to AMO in the following reactions:
 HAO reaction, 2 electrons gained:
@@ Line 16: / Line 13: @@
 '''NH<sub>3</sub> + O<sub>2</sub> + cycAX<sub>red</sub> + 2 H<sup>+</sup> &rarr; NH<sub>2</sub>OH + H<sub>2</sub>O + cycAX<sub>ox</sub>'''
+===Usage and Biology===
+When highly expressed in ''E.coli'' the cells turn dark red. If grown on media with supplementation of d-aminolevulinic acid (ALA) it tends to get a darker color because of the extra heme incorporated.
 <span class='h3bb'>Sequence and Features</span>