Difference between revisions of "Part:BBa J37027:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J37027 short</partinfo>
 
<partinfo>BBa_J37027 short</partinfo>
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===Source===
 
===Source===
  
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From Regestry:
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From Registry:
 
BBa_B0015
 
BBa_B0015
 
BBa_I13521
 
BBa_I13521

Revision as of 15:36, 1 August 2006

Pops Blocker (Cre/Lox system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
    Illegal AgeI site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used specific lox sites to make this irreversable


Source

From Registry: BBa_B0015 BBa_I13521

Lox Sites: Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH

References