Difference between revisions of "Part:BBa K1045000"
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===Usage and Biology===
 
===Usage and Biology===
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The DarR operator is a 14 bp palindromic DNA sequence discovered in ''Mycobacterium smegmatis''. The DarR operator can be bound by the HTH-transcription factor DarR. In ''M. smegmatis'', DarR and the DarR operator control the expression of the darR gene (auto-regulation) and other genes predicted to be involved in different processes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene. (Zhang et al., 2013)
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Revision as of 13:55, 28 September 2013

DarR operator

This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed.
The important information: The operator is not in the pSB1C3 Vector but instead in the pBluescript-KS plasmid. This plasmid provides an ampicillin resistance. Furthermore, it has got a pUC origin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]