Difference between revisions of "Part:BBa K1033104:Experience"

Revision as of 13:15, 28 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1033104

This part was sucessfully cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034 and biobrick restriction sites. As it contained an illegal SpeI site, mutagenisation was performed, with sucess. The sequencencing was done at GATC biotech and Uppsla Genome center. We were unsucessful in assembling any promoter to the mutagenized construct, and as such could not further characterize the part.

User Reviews

UNIQ9bee19817e0642bf-partinfo-00000000-QINU UNIQ9bee19817e0642bf-partinfo-00000001-QINU

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 ===Applications of BBa_K1033104===
-This part was sucessfully cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034. As it contained an ilegal SpeI site, mutagenisation was sucessfully performed. The sequencencing was done at GATC biotech and Uppsla Genome center. We were unsucessful in assembling any promoter to the mutagenized construct, and as such could not further characterize the part.
+This part was sucessfully cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034 and biobrick restriction sites. As it contained an illegal SpeI site, mutagenisation was performed, with sucess. The sequencencing was done at GATC biotech and Uppsla Genome center. We were unsucessful in assembling any promoter to the mutagenized construct, and as such could not further characterize the part.
 ===User Reviews===