Difference between revisions of "Part:BBa K1073022:Experience"
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'''iGEM Team Braunschweig 2013:''' Emission spectrum of chromoprotein eforRed. The chromoprotein does show significant emission and can be detected by its fluorescence. | '''iGEM Team Braunschweig 2013:''' Emission spectrum of chromoprotein eforRed. The chromoprotein does show significant emission and can be detected by its fluorescence. | ||
| − | '''Emission maximum of eforRed:''' 605 | + | '''Emission maximum of eforRed:''' 605 nm |
Revision as of 12:21, 28 September 2013
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Applications of BBa_K1073022
When eforRed is expressed, the colonies on agar plates as well as liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to distinguish two different strains when cultivation in a mixed culture.
User Reviews
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iGEM Team Braunschweig 2013 |
When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoprotein during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm.
iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm. Absorption maximum of eforRed: 534 nm
iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. The chromoprotein does show significant emission and can be detected by its fluorescence. Emission maximum of eforRed: 605 nm
iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light. |
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