Difference between revisions of "Part:BBa K1073013"
 
Line 2: Line 2:
 
<partinfo>BBa_K1073013 short</partinfo>
 
<partinfo>BBa_K1073013 short</partinfo>
  
−
This device is a combination of <partinfo>BBa_K1073003</partinfo> and <partinfo>BBa_K1073000</partinfo> and mediates a inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of ''Pseudomonas aeruginosa'' followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.  
+
This device is a combination of <partinfo>BBa_K1073003</partinfo> and <partinfo>BBa_K1073000</partinfo> and mediates an inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of ''Pseudomonas aeruginosa'' followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.  
  
  

Latest revision as of 11:10, 28 September 2013

Inducible ampicillin resistance cassette (regulated by LasR + N-3-oxododecanoyl-HSL)

This device is a combination of BBa_K1073003 and BBa_K1073000 and mediates an inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.


Usage and Biology

The promoter is activated in the presence of LasR in combination with the autoinducer molecule N-3-oxododecanoyl-homoserine lactone produced by LasI. The expression of the beta-lactamase can therefore be induced and tuned by supplying LasR and N-3-oxododecanoyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073034.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]