Difference between revisions of "Part:BBa K1073013:Experience"

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<I>iGEM Team Braunschweig 2013</I>
 
<I>iGEM Team Braunschweig 2013</I>
 
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This device was successfully employed in <partinfo>BBa_K1073034</partinfo> to mediate ampicillin resistance in the presence of LasR and N-3-oxododecanoyl-HSL. Due to the leakiness of the promoter cells also grow when N-3-oxododecanoyl-HSL is not present. Uninduced activity of the promoter differs depending on strain and media used.
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This device was successfully employed in <partinfo>BBa_K1073034</partinfo> to mediate ampicillin resistance in the presence of LasR and N-3-oxododecanoyl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithoug N-buturyl-HSL. Uninduced activity of the promoter differs depending on strain and media used.
 
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Latest revision as of 11:03, 28 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073013

iGEM Team Braunschweig 2013 used this device for the construction of BBa_K1073034. It was used in order to induce growth in the presence of LasR and N-3-oxododecanoyl-HSL in ampicillin containing media.

User Reviews

UNIQaa74d7dba4cb61a7-partinfo-00000001-QINU

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iGEM Team Braunschweig 2013

This device was successfully employed in BBa_K1073034 to mediate ampicillin resistance in the presence of LasR and N-3-oxododecanoyl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithoug N-buturyl-HSL. Uninduced activity of the promoter differs depending on strain and media used.

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UNIQaa74d7dba4cb61a7-partinfo-00000004-QINU