Difference between revisions of "Part:BBa K1065300"
Line 3: Line 3:
  
 
This part is composed by a pLacI and K952003. The part does not work because it lacks the RBS between the Pfixk2 and the amilGFP
 
This part is composed by a pLacI and K952003. The part does not work because it lacks the RBS between the Pfixk2 and the amilGFP
−
 
+
We doing a mutagenesis at the part to insert RBS before the amilGFP and the new resulting parts is named K1065302.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:06, 28 September 2013

R0010-YF1-FixJ-PfixK2-amilGFP

This part is composed by a pLacI and K952003. The part does not work because it lacks the RBS between the Pfixk2 and the amilGFP We doing a mutagenesis at the part to insert RBS before the amilGFP and the new resulting parts is named K1065302. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 950
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1755
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1519
    Illegal BsaI.rc site found at 383