Difference between revisions of "Part:BBa K1189009:Design"
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Note: The Bsa1 cut site has | + | Note: The Bsa1 cut site has NOT been removed. This part has been designed in the RFC 25 Freiburg fusion backbone and can be used to make fusion proteins. The ATG codon is incorporated in the backbone before the NgoMIV restriction cut site. |
| − | + | ||
| + | ======== | ||
TCT: Original serine - mutated to TCC: serine | TCT: Original serine - mutated to TCC: serine | ||
| + | ======== | ||
Mutagenesis Primers | Mutagenesis Primers | ||
| + | ======== | ||
F: 5’GGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGC 3’ | F: 5’GGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGC 3’ | ||
| + | ======== | ||
R: 5’GCTGCAATGATACCGCGGGACCCACGCTCACCGGCTCC 3’ | R: 5’GCTGCAATGATACCGCGGGACCCACGCTCACCGGCTCC 3’ | ||
Revision as of 07:06, 28 September 2013
Beta-lactamase with His Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note: The Bsa1 cut site has NOT been removed. This part has been designed in the RFC 25 Freiburg fusion backbone and can be used to make fusion proteins. The ATG codon is incorporated in the backbone before the NgoMIV restriction cut site.
==
TCT: Original serine - mutated to TCC: serine
==
Mutagenesis Primers
==
F: 5’GGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGC 3’
==
R: 5’GCTGCAATGATACCGCGGGACCCACGCTCACCGGCTCC 3’