Difference between revisions of "Part:BBa K1189031"
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<partinfo>BBa_K1189031 short</partinfo> | <partinfo>BBa_K1189031 short</partinfo> | ||
| − | + | This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification. | |
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| + | <img src="https://static.igem.org/mediawiki/parts/5/51/YYC_2013_PlacI_%2B_His_%2B_TALE_%2B_Blac.jpg"> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 05:39, 28 September 2013
TALE-A linked to beta-lactamase with a his tag under a lacI promoter
This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1922
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2651
Illegal AgeI site found at 677
Illegal AgeI site found at 1258 - 1000COMPATIBLE WITH RFC[1000]