Difference between revisions of "Part:BBa K1129007"
Line 5: Line 5:
 
Repeat/Spacer Region
 
Repeat/Spacer Region
 
Confers resistance to T4 page upon those strains containing Cas9 and TracrRNA.
 
Confers resistance to T4 page upon those strains containing Cas9 and TracrRNA.
 +
 +
This spacer is computationally verified to targets all of
 +
AR1 HX01 ime09 RB14 RB32 RB51 RB69 T4 vB_EcoM_ACG-C40 wV7 phage genomes.
  
 
Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR
 
Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR

Revision as of 04:38, 28 September 2013

T4 Repeat/Spacer Region of T4 Bacteriophage


Repeat/Spacer Region Confers resistance to T4 page upon those strains containing Cas9 and TracrRNA.

This spacer is computationally verified to targets all of AR1 HX01 ime09 RB14 RB32 RB51 RB69 T4 vB_EcoM_ACG-C40 wV7 phage genomes.

Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]