Difference between revisions of "Part:BBa K1162005"

Latest revision as of 04:08, 28 September 2013

Scygonadin antimicrobial peptide from the horseshoe crab (Scylla serrata)

Scygonadin is an anionic antimicrobial peptide produced in a species of mangrove crab to prevent bacterial invasion. It is the primary defense against dangerous microbes that thrive in marine environments. Scygonadin has been shown to be effective in inhibiting both gram-positive and gram-negative bacteria including M. leteus, S. aureus, C. glutamicum, and A. hydrophila. This AMP has been previously studied for its production via recombinant DNA in E. coli using a pTrc-CKS vector and a C-terminus 6x His-tag. No studies were done with a 10x His-Tag.

Design

This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1162005 short</partinfo>
-An anionic antimicrobial peptide.
+Scygonadin is an anionic antimicrobial peptide produced in a species of mangrove crab to prevent bacterial invasion. It is the primary defense against dangerous microbes that thrive in marine environments. Scygonadin has been shown to be effective in inhibiting both gram-positive and gram-negative bacteria including <i>M. leteus, S. aureus, C. glutamicum, and A. hydrophila</i>. This AMP has been previously studied for its production via recombinant DNA in E. coli using a pTrc-CKS vector and a C-terminus 6x His-tag. No studies were done with a 10x His-Tag.
+===Design===
+This part was codon optimized for <i>E. coli</i> using the Life Technologies GeneArt &#174; software program. By design, this part is [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and contains a Methionine(atg) residue at the start of the coding region.  Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.
 <!-- Add more about the biology of this part here