Difference between revisions of "Part:BBa K1064002"
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We use GFP generator (BBa_E0840) under the control pSOS promotor (BBa_J22106) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064002). pSOS promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein | We use GFP generator (BBa_E0840) under the control pSOS promotor (BBa_J22106) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064002). pSOS promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein | ||
| − | will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy, while the control (without UV irradiation) doesn't show the green fluorescence [[File:Psos.jpg]] | + | will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy, while the control (without UV irradiation) doesn't show the green fluorescence [[File:Psos.jpg]]. |
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| + | We also characterized the performance of [[BBa_K1064002]] and [[BBa_K1064003]] in response toward DNA damage [[File:Fold GFP.jpg]] | ||
Latest revision as of 03:42, 28 September 2013
We use GFP generator (BBa_E0840) under the control pSOS promotor (BBa_J22106) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064002). pSOS promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein
will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy, while the control (without UV irradiation) doesn't show the green fluorescence 
.
We also characterized the performance of BBa_K1064002 and BBa_K1064003 in response toward DNA damage 