Difference between revisions of "Part:BBa K1129043"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1129043 short</partinfo>
 
<partinfo>BBa_K1129043 short</partinfo>
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Arab.+rbs+4-CMH+term
 
Arab.+rbs+4-CMH+term
  
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4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enyzme the biosynthetic pathway of vanillin from the amino acid tyrosine. The enzyme is under arabinose inducible promoter and a terminator.<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 03:38, 28 September 2013

4-coumarate 3-hydroxylase under arabinose promoter

Arab.+rbs+4-CMH+term

4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enyzme the biosynthetic pathway of vanillin from the amino acid tyrosine. The enzyme is under arabinose inducible promoter and a terminator. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 203
    Illegal NgoMIV site found at 953
    Illegal NgoMIV site found at 1126
    Illegal NgoMIV site found at 1528
    Illegal NgoMIV site found at 1532
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727