Difference between revisions of "Part:BBa K1129046"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1129046 short</partinfo>
 
<partinfo>BBa_K1129046 short</partinfo>
Line 5: Line 4:
 
Const.+rbs+4-CMH
 
Const.+rbs+4-CMH
  
−
<!-- Add more about the biology of this part here
+
4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enyzme the biosynthetic pathway of vanillin from the amino acid tyrosine. <!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 03:34, 28 September 2013

4-coumarate 3-hydroxylase under pTET constitutive promoter

Const.+rbs+4-CMH

4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enyzme the biosynthetic pathway of vanillin from the amino acid tyrosine. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 100
    Illegal NgoMIV site found at 108
    Illegal NgoMIV site found at 858
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 1433
    Illegal NgoMIV site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 632