Difference between revisions of "Part:BBa K1166000"
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==Characterization== | ==Characterization== | ||
− | <p>After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. | + | <p>After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate. |
[[File:HIP-1 characterization.jpg|800 px|]] | [[File:HIP-1 characterization.jpg|800 px|]] |
Revision as of 03:34, 28 September 2013
FNR-HIP1
HIP-1 drives gene expression under both acute and chronic hypoxia, but not under normoxia (Mengesha, et al., 2006). This part is composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.
Characterization
After 4 days in culture the expression of GFP controlled by the pHG the fluorescence was measured in a 96-well plate.
References
Mengesha A, Dubois L, Lambin P, Landuyt W, Chiu RK, Wouters BG, Theys J. (2006). Development of a flexible and potent hypoxia-inducible promoter for tumor-targeted gene expression in attenuated Salmonella. Cancer Biol Ther. 5(9):1120-8.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]