Difference between revisions of "Part:BBa K1087015:Experience"
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| + | http://2013.igem.org/Team:SCU_China/Project | ||
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| + | We transformed BBa_K1087015, BBa_K1087021into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. We used fluorescence microscope to observe the fluorescence at 16h, and we also used Thermo varioskan flash to quantitatively measured the fluorescence intensity at 16h,18h, and 19h, respectively. | ||
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| + | According to the data,the ribolock BBa_K1087004 works well, and its fluorescence intensity was only 2.6531% compared to positive control even at 19h, while that of lock & key (BBa_K1087021) was 91.7138% at 19h. The results suggest the key BBa_K145215 can hugely improve the expression level of the lock BBa_K1087004. | ||
Latest revision as of 03:33, 28 September 2013
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1087015
User Reviews
UNIQ273ba56d09163349-partinfo-00000000-QINU UNIQ273ba56d09163349-partinfo-00000001-QINU
http://2013.igem.org/Team:SCU_China/Project
We transformed BBa_K1087015, BBa_K1087021into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. We used fluorescence microscope to observe the fluorescence at 16h, and we also used Thermo varioskan flash to quantitatively measured the fluorescence intensity at 16h,18h, and 19h, respectively.
According to the data,the ribolock BBa_K1087004 works well, and its fluorescence intensity was only 2.6531% compared to positive control even at 19h, while that of lock & key (BBa_K1087021) was 91.7138% at 19h. The results suggest the key BBa_K145215 can hugely improve the expression level of the lock BBa_K1087004.