Difference between revisions of "Part:BBa K1129027"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1129027 short</partinfo> | <partinfo>BBa_K1129027 short</partinfo> | ||
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Arab+sense tracRNA+term | Arab+sense tracRNA+term | ||
+ | There are several pathways of CRISPR activation, one of which requires a tracrRNA which plays a role in the maturation of crRNA. TracrRNA is complementary to and base pairs with a pre-crRNA forming an RNA duplex. | ||
+ | http://en.wikipedia.org/wiki/TracrRNA | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 03:25, 28 September 2013
sense tracRNA under arabinose promoter
Arab+sense tracRNA+term
There are several pathways of CRISPR activation, one of which requires a tracrRNA which plays a role in the maturation of crRNA. TracrRNA is complementary to and base pairs with a pre-crRNA forming an RNA duplex. http://en.wikipedia.org/wiki/TracrRNA Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]