Difference between revisions of "Part:BBa K1129037"

Revision as of 03:24, 28 September 2013

4-coumarate-CoA ligase (4CL) under arabinose promoter

The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.

Resources

http://www.uniprot.org/uniprot/Q1H8P3

http://en.wikipedia.org/wiki/4-Coumarate-CoA_ligase

http://www.genome.jp/kegg-bin/show_pathway?map00940

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
Illegal NheI site found at 1242
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1809
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1441

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1129037 short</partinfo>
-Arab.+rbs+4-CL+term.
+The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.
+'''Resources'''
+http://www.uniprot.org/uniprot/Q1H8P3
+http://en.wikipedia.org/wiki/4-Coumarate-CoA_ligase
+http://www.genome.jp/kegg-bin/show_pathway?map00940
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 25: @@
 <partinfo>BBa_K1129037 parameters</partinfo>
 <!-- -->
-The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.
-'''Resources'''
-http://www.uniprot.org/uniprot/Q1H8P3
-http://en.wikipedia.org/wiki/4-Coumarate-CoA_ligase
-http://www.genome.jp/kegg-bin/show_pathway?map00940