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Utah State iGEM team 2013 had good results using this 10x His-Tag for the purification of antimicrobial spider silk from E. coli [https://parts.igem.org/Part:BBa_K1162306 BBa_K1162306].

Latest revision as of 03:14, 28 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K844000

User Reviews

UNIQ7d60155695f010af-partinfo-00000000-QINU

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2012 Utah_State iGEM Team

This part functions as expected: it is capable of binding to Nickel resin columnns with high affinity, and it capable of being recognized by histidine-tag specific antibodies and dyes. See the main part page for gel photo and details.

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UNIQ7d60155695f010af-partinfo-00000002-QINU UNIQ7d60155695f010af-partinfo-00000003-QINU

Utah State iGEM team 2013 had good results using this 10x His-Tag for the purification of antimicrobial spider silk from E. coli BBa_K1162306.