Difference between revisions of "Part:BBa K1166003"
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A biobrick that includes the TAT signal peptide that can be fused to any protein for an easy internalization into mammalian cells (Vivès, et al., 1997). It includes a 6x His-tag for easy purification. | A biobrick that includes the TAT signal peptide that can be fused to any protein for an easy internalization into mammalian cells (Vivès, et al., 1997). It includes a 6x His-tag for easy purification. | ||
TAT is a cell penetrating peptide (CPP) derived from the transactivator of transcription from HIV and has been used to internalize a range of large and small molecules from peptides, DNA and antibodies, to liposomes. | TAT is a cell penetrating peptide (CPP) derived from the transactivator of transcription from HIV and has been used to internalize a range of large and small molecules from peptides, DNA and antibodies, to liposomes. | ||
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==References== | ==References== | ||
Revision as of 03:10, 28 September 2013
His-TAT
A biobrick that includes the TAT signal peptide that can be fused to any protein for an easy internalization into mammalian cells (Vivès, et al., 1997). It includes a 6x His-tag for easy purification. TAT is a cell penetrating peptide (CPP) derived from the transactivator of transcription from HIV and has been used to internalize a range of large and small molecules from peptides, DNA and antibodies, to liposomes.
References
Vivès E, Brodin P, Lebleu B. (1997). A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J Biol Chem. 272(25):16010-7
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]