Difference between revisions of "Part:BBa K1087004:Experience"
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We assemblied BBa_K1087004 with mRFP to create BBa_K1087015, and we ligate BBa_K1087015 with RiboKey BBa_K145215 to create BBa_K1087021. | We assemblied BBa_K1087004 with mRFP to create BBa_K1087015, and we ligate BBa_K1087015 with RiboKey BBa_K145215 to create BBa_K1087021. | ||
| + | Then, we transformed BBa_K1087015, BBa_K1087021 into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. | ||
| + | All of the constructs were expressed in high copy plasmid PSB1X3. | ||
| + | |||
| + | Data: | ||
| + | Fluorescence | ||
| + | K1087015 K1087021 I13521 | ||
| + | 16h 0.089 6.8595 14.849 | ||
| + | 18h 0.486 16.011 18.363 | ||
| + | 19h 0.629 21.7435 23.708 | ||
Revision as of 03:08, 28 September 2013
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UNIQeb9c7b4d8430df5c-partinfo-00000000-QINU UNIQeb9c7b4d8430df5c-partinfo-00000001-QINU We assemblied BBa_K1087004 with mRFP to create BBa_K1087015, and we ligate BBa_K1087015 with RiboKey BBa_K145215 to create BBa_K1087021. Then, we transformed BBa_K1087015, BBa_K1087021 into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. All of the constructs were expressed in high copy plasmid PSB1X3.
Data: Fluorescence K1087015 K1087021 I13521 16h 0.089 6.8595 14.849 18h 0.486 16.011 18.363 19h 0.629 21.7435 23.708